A PRACTICAL BOOK OF PHARMACOLOGY – 2 (Third Year BPharm – Semester 5)

Experiment No. 1 to Experiment No. 17

1. Study of laboratory appliances used in experimental pharmacology.
2. Physiological Salt Solutions (PSS).
3. Study the effect of drugs on frog heart.
4. Recording of rat’s BP, Heart Rate and ECG.
5. Study of diuretic activity of drug using rat.
6. Dose response curve of agonist using guinea pig ileum preparation.
7. Determination of the strength of unknown sample by matching bioassay by using suitable tissue preparation.
8. Determination of the strength of unknown sample by interpolation bioassay by using suitable tissue preparation.
9. Three point bio-assay of acetylcholine using rat ileum preparation.
10. Determination of the strength of unknown sample by multiple point bioassay by using suitable tissue preparation.
11. Determination of various antagonists PA₂ values using suitable isolated tissue preparations.
12. Determination of pD₂ values using guinea pig ileum.
13. Study of the property of antagonist or potentiating agents on DRC of agonist using suitable isolated tissue preparation.
14. Study of the anti-inflammatory property of drugs using rat paw edema method.
15. Study of the analgesic property of drugs using thermal methods.
16. Screening of analgesic activity of diclofenac by acetic acid induced writhing in mice.
17. Study of the analgesic property of drugs using thermal methods.